Applications

Cofactor can produce a large variety of libraries for multiple applications and from a wide range of sample types. Several examples are outlined in greater detail below. Additional libraries may be possible upon request.

Fragment View »



Whole genome characterization by single-pass shotgun sequencing of fragments from total DNA, PCR products, etc.

Paired-end View »



Whole genome characterization by shotgun sequencing from both ends of DNA fragments with ~200bp inserts. Specialty large inserts libraries are available upon request for an additional charge.

ChIP-Seq View »



Discovery & quantitation of protein-DNA interactions by sequencing DNA from immunoprecipitations.

MicroRNA View »



Discovery & quantitation of novel microRNAs and isoforms by sequencing cDNAs of microRNAs isolated from total RNA.

TranscriptomeView»



Quantitative whole transcriptome profiling (RNA-seq) by sequencing cDNAs constructed from messenger RNA isolated from total RNA.

Bisulfite View »



Genome methylation profiling by sequencing DNA fragments bisulfite treated to convert non-methylated C’s into U’s.



Capture View »


Single-nucleotide polymorphism and insertion/deletion detection by targeted selection and sequencing of discreet genomic regions of interest.



Bisulfite

Genome methylation profiling by sequencing DNA fragments bisulfite treated to convert non-methylated C’s into U’s

Using bisulfite conversion, we can determine the positions of all methylated C’s in the genome. This chemical process modifies non-methylated C’s so that following PCR they are sequenced as A’s. By mapping these reads back to the genome using highly-specialized alignment tools, we can observe originally methylated C’s by looking for columns of our bisulfite-induced C to A “mismatches.”

DNA methylation is just one epigenomic modification that can be revealed by
sequencing. For histone methylation, see ChIP-Seq.





References

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