Applications

Cofactor can produce a large variety of libraries for multiple applications and from a wide range of sample types. Several examples are outlined in greater detail below. Additional libraries may be possible upon request.

Fragment View »



Whole genome characterization by single-pass shotgun sequencing of fragments from total DNA, PCR products, etc.

Paired-end View »



Whole genome characterization by shotgun sequencing from both ends of DNA fragments with ~200bp inserts. Specialty large inserts libraries are available upon request for an additional charge.

ChIP-Seq View »



Discovery & quantitation of protein-DNA interactions by sequencing DNA from immunoprecipitations.

MicroRNA View »



Discovery & quantitation of novel microRNAs and isoforms by sequencing cDNAs of microRNAs isolated from total RNA.

TranscriptomeView»



Quantitative whole transcriptome profiling (RNA-seq) by sequencing cDNAs constructed from messenger RNA isolated from total RNA.

Bisulfite View »



Genome methylation profiling by sequencing DNA fragments bisulfite treated to convert non-methylated C’s into U’s.



Capture View »


Single-nucleotide polymorphism and insertion/deletion detection by targeted selection and sequencing of discreet genomic regions of interest.



Fragment

Whole genome characterization by single-pass shotgun sequencing of fragments from total DNA, PCR products, etc

Fragment reads are the most fundamental type of sequencing from DNA samples. Primarily, you use fragment reads to detect SNPs and small indels in previously sequenced genomes of any size.

We recommend a minimum coverage of 10X - 10 times the genome size - for haploid genomes and at least 20X for diploids. This provides enough reads to (1) overcome any substantial gaps due to the random placement of reads, (2) overcome sequencing errors in individual reads, and (3) sample sufficiently from both alleles of a diploid genome.

Fragment libraries are typically constructed from 5 micrograms of total DNA.