Applications

Cofactor can produce a large variety of libraries for multiple applications and from a wide range of sample types. Several examples are outlined in greater detail below. Additional libraries may be possible upon request.

Fragment View »



Whole genome characterization by single-pass shotgun sequencing of fragments from total DNA, PCR products, etc.

Paired-end View »



Whole genome characterization by shotgun sequencing from both ends of DNA fragments with ~200bp inserts. Specialty large inserts libraries are available upon request for an additional charge.

ChIP-Seq View »



Discovery & quantitation of protein-DNA interactions by sequencing DNA from immunoprecipitations.

MicroRNA View »



Discovery & quantitation of novel microRNAs and isoforms by sequencing cDNAs of microRNAs isolated from total RNA.

TranscriptomeView»



Quantitative whole transcriptome profiling (RNA-seq) by sequencing cDNAs constructed from messenger RNA isolated from total RNA.

Bisulfite View »



Genome methylation profiling by sequencing DNA fragments bisulfite treated to convert non-methylated C’s into U’s.



Capture View »


Single-nucleotide polymorphism and insertion/deletion detection by targeted selection and sequencing of discreet genomic regions of interest.



SmallRNA

Discovery & quantitation of novel microRNAs and isoforms by sequencing cDNAs of microRNAs isolated from total RNA

Similar to RNA-seq, microRNA sequencing samples quantitatively from the entire small RNA population of the cell, including most RNAs smaller than tRNAs. From this data we can simultaneously discover the expressed microRNA population of the sample as well as build an expression profile that can be compared to other samples.

Submit 20 micrograms of total RNA to Cofactor and we will extract the microRNAs, make the cDNAs, construct libraries, sequence and analyze.